Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Tumors were embedded and frozen in OCT freezing compound. Frozen sections were mounted on laser capture microdissection slides and stained with the Arcturus Histogene Frozen Section Staining Kit. Laser capture microdissection of tumor regions was performed on a Leica Microsystems LMD7000. RNA was isolated with the Arcturus PicoPure RNA Isolation Kit. cDNA libraries were prepared from Illumina RNA using the TruSeq RNA Sample Prep Kit v2 per manufacturer's protocol.